Get Full Essay Get access to this section to get all help you need with your essay and educational issues.
Get Full Essay Get access to this section to get all help you need with your essay and educational issues. The topic of this research involved the occurrence of genetic transformation in bacteria E. More specifically, a previously prepared pGLO plasmid—which consisted of the gene to be cloned—was used to transform non-pathogenic bacteria.
Essentially, we wanted to determine the conditions of the bacteria that would glow. We essentially made the required transformed solutions—and the controls—swiped them on the agar plate, and then observed to see whether or not bacteria colonies grew and whether or not they glowed.
Our data fully supported our hypothesis.
We can thus conclude that bacteria can take in foreign DNA through the process of transformation and that this foreign DNA can fundamentally change the bacteria ex: Future research can involve inserting other pieces of DNA into bacteria from different organisms, making the bacteria take on various phenotypic characteristics.
Genetic transformation is one of the most important processes in biotechnology. Essentially, genetic transformation involves the process where a cell in this lab, a bacterial cell takes up foreign DNA from its surroundings and incorporates it into its own DNA.
This gene transfer is accomplished with the aid of a plasmid, a naturally-occuring small piece of circular DNA in bacteria. In biotechnology laboratories, plasmids are transformed. Then, this gene to be transferred is forged into the plasmid in this lab we used previously made plasmids called pGLO, containing a bioluminescent gene and a gene for ampicillin resistance.
In the end—in order to find out which bacteria received the new gene—the transformed solution can be incubated on agar gel. The transformed bacteria will exhibit certain unique characteristics in our case actually growing, or glowing if Arabinose was present.
For this experiment, the following materials were used: Next, we used two sterile loops to transfer E. We incubated both micro test tubes on ice for 10 minutes. We let these tubes sit for ten minutes at room temperature. Finally, each transformation solution was prepared.
Lastly, we put our plates in an incubator so bacteria could grow, and observed them the next day under UV light. Coli bacteria, only some of which would take in this plasmid. In the agar plate with just LB and -DNA, a bacteria lawn was present bacteria were everywhere, but they did not glow.
Coli via pGLO plasmids fusing into the bacteria. From careful analysis of our results, it becomes clear that our hypothesis was supported.Glowing Transformations Essay Sample.
The whole doc is available only for registered users OPEN DOC. Pages: Word count: For example, if the ampicillin in the media was contaminated, it could have weakened, thus causing a very low to no susceptibility in the bacteria.
which both the partial purpose and same bacteria used in this lab. We will then place the loop into calcium chloride and twirl it rapidly, releasing the bacteria. Then, using a pipette, we will add 10µl of the pBestLuc solution to the mixture. We will write a custom essay sample on Glowing Bacteria Lab specifically for you.
From this lab, we can conclude that bacteria–through the process of transformation–are able to take up foreign DNA (such as in the form of a pGLO plasmid) and express it . Biotechnology Bacterial Transformation Lab: The effects of pGLO DNA on E. coli Method Introduction Bacteria transformation is the process of a bacterium absorbing and integrating naked DNA located on the surface of their membrane.
When we rehydrate the plasmids, the gene necessary to produce glowing bacteria, along with several genes that are part of the control for the experiment will be contained in a neat package that the bacteria will take up on its own.
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Bacterial Transformation Lab Report Essay. A+. Pages:5 Words This is just a sample. All the results came out as they were supposed to.
The +pGLO with LB/Amp/Ara has glowing bacteria due to the transformation it went through. If this lab was done over again, it would most likely be done on a larger scale.
Example, maybe .